Human Anti-IL2 Recombinant Antibody (PABC-506)
CAT#: PABC-506
This product is a recombinant Human antibody that can recognize IL2.
Tested Data
Gene Expression
ELISA
Figure 1 Anti-IL2 Recombinant Antibody (PABC-506) in ELISA
ELISA analysis of PABC-506 was performed by coating with human IL2 protein (His tag). Then blocked with BSA and incubated with PABC-506. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-IL2 Recombinant Antibody (PABC-506) in WB
Western blot analysis of PABC-506 was performed by loading human IL2 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABC-506 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.
DB
Figure 3 Anti-IL2 Recombinant Antibody (PABC-506) in DB
Dot Blot analysis of PABC-506 was performed by coating with human IL2 protein (His tag).
PABC-506 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
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Normal Tissue
Figure 1 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10310/26644_A_7_3.jpg
Normal Tissue
Figure 2 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10310/26644_A_8_5.jpg
Normal Tissue
Figure 3 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10310/26644_A_6_6.jpg
RNA Expression
Figure 4 RNA cell line category: Not detected
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000109471-IL2
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Specifications
- Host Species
- Human
- Type
- Human IgG1, λ
- Specificity
- Human IL2
- Species Reactivity
- Human
- Applications
- Inhib, ELISA, FC, FuncS
- Related Disease
- Autoimmune diseases and graft-versus-host disease
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Inhibition, Flow Cytometry and Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-506, RRID: AB_3111595)
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Related Diseases
Autoimmune Thyroid Disease
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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